p16基因在肺癌中突变和表达改变的研究

The mutation and down-expression of p16 gene in human lung cancer

摘要: 目的检测非小细胞肺癌ｐ１６基因蛋白表达及其第２外显子突变，并探讨其与非小细胞肺癌的临床病理类型和预后的关系。方法用Ｓ－Ｐ免疫组化法对ｐ１６基因蛋白表达进行对比检测，并用ＰＣＲ－ＳＳＣＰ银染技术检测ｐ１６基因第２外显子的突变情况。结果非小细胞肺癌中ｐ１６蛋白表达阳性率５２．５％，肺部非癌性病变阳性率９２．５％，两者比较差异有显著性意义（Ｐ＜０．０５）；非小细胞肺癌中Ｐ１６基因第２外显子突变率为５％，而肺部非癌性病变中无一例突变。结论ｐ１６蛋白表达下调在非小细胞肺癌的发生发展中起重要作用，对判断其预后有意义，提示Ｐ１６／ＭＴＳ１基因在非小细胞肺癌发生中具有一定的作用。

Abstract: Objective To detect pl6 gene mutation and expression in human non-small cell lung cancer (NSCLS), and to study the relationship between p16 expression and clinicpathologic features and progno- sis. Methods With S-P immunohistochenistry method, we investigated piG protein expression. Mutation in exon 2 of p16/MTSJ gene was screened by PCR-SSCP analyzing with non-isotope silve staining method. Results The positive rate of piG expression in NSCLC and lung benigh diseases was...