温热顺铂对肝癌细胞的杀伤效应及其诱导凋亡的研究

Studies on the effect of cell killing and relationship between heated-CDDP and induction of apoptosis in Hca-F

摘要: 目的探讨温热ＣＤＤＰ对Ｈｃａ－Ｆ细胞的生长抑制、温度与药效的关系以及诱导凋亡的作用。方法采用ＭＴＴ法，透射电镜，ＤＮＡ电泳以及流式细胞术分析法。结果ＣＤＤＰ对Ｈｃａ－Ｆ细胞有明显的细胞毒性，其ＩＣ５０值为０．５７μｇ／ｍｌ。ＣＤＤＰ合并高温（＞４１℃）加热明显提高了ＣＤＤＰ的杀伤力，二者之间存在着协同增敏作用。其作用强度呈现对药物浓度、作用时间与加热温度的正相关性，且在一定浓度范围内以谤导肿瘤细胞凋亡的发生为主。结论加热（＞４１℃）顺铂提高了顺铂的细胞毒性。诱导细胞凋亡的发生是其抗肿瘤作用的主要机制之一。

Abstract: Objective To determine the effect of inhibition of cell growth of hyperthermia in combination with CDDP on Hca-F cell lines, and the relationship between temperature and medical effect as well as re- lationship between heated-DDP and induction of apoptosis in Hca-F. Methods Observing the relationship between temperature and effect of CDDP by typanblue assay and transmission electron microscopic tech- nique and flow cytometry as well as DNA fragmentation. Results Heating-C...