用IgH、TCR基因重排技术检测疑难淋巴组织增生性病变

Detecting Problematic Proliferative Lesions of Lymph Tissue by IgH and TCR Gene Rearrangement Technique

摘要: 目的探讨IgH、TCR基因重排技术检测疑难淋巴组织增生性病变的意义。方法采用IgHTCR-β、TCR-γ基因重排标志检测,36例经常规HE、免疫组化不能诊断的疑难淋巴组织增生性病变,并进行克隆性分析。结果29例淋巴组织增生性病变呈克隆阳性,其中IgH、TCR双重排阳性者为1例,IgH单一阳性8例,TCR单阳性为8例。克隆阳性病例与免疫组化结果一致的为24/29例,7例阴性者,4例为反应性增生,2例经随诊为猫抓病淋巴结炎,1例为不典型增生。结论IgH、TCR基因重排技术对于疑难淋巴组织增生性病变的诊断和鉴别诊断有较大的意义。

Abstract: Objective To investigate the significance in diagnosis of problematic proliferative lesions of lymph tissue by IgH, TCR gene rearrangement technique. Methods Thirty-six cases of proliferative lesions of lymph tissue with diagnostic difficulty by routine HE and immunohistochemistry, were analyzed in detail by PCR-based IgH, TCR gene rearrangement technique to further determine the diagnosis. 20 cases of previously diagnosed lymphoma and 10 reactive lymphadenitis were used as a positive control and negative co...