Effects of Circular RNA hsa_circ_0001922 on Proliferation, Migration and Invasion of Prostate Cancer Cells and Its Potential Molecular Mechanism
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摘要:目的
探讨环状RNA hsa_circ_0001922对前列腺癌细胞PC-3增殖、迁移和侵袭的影响及其潜在分子机制。
方法qRT-PCR、RNA FISH分别检测hsa_circ_0001922在前列腺癌细胞PC-3中的表达水平及定位。靶向抑制hsa_circ_0001922后，使用克隆形成、Transwell实验及划痕实验检测PC-3细胞增殖、迁移和侵袭的能力；qRT-PCR、Western blot检测抑制hsa_circ_0001922后EMT通路相关分子表达水平。
结果环状RNA hsa_circ_0001922在前列腺癌细胞PC-3中表达上升（P < 0.01）且存在于细胞质和细胞核中。靶向抑制hsa_circ_0001922后，细胞增殖、迁移和侵袭能力明显减弱（P < 0.05），E-cadherin mRNA表达水平上升（P < 0.05），而Vimentin mRNA水平下降（P < 0.05）。Western blot检测结果与上述一致（均P < 0.05）。
结论环状RNA hsa_circ_0001922可能通过EMT途径促进PC-3细胞的增殖、迁移及侵袭。
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关键词:
- 前列腺癌 /
- hsa_circ_0001922 /
- 增殖 /
- 侵袭 /
- EMT
Abstract:ObjectiveTo investigate the effect of circular RNA hsa_circ_0001922 on the proliferation, migration and invasion of prostate cancer cell PC-3 and its underlying molecular mechanism.
MethodsqRT-PCR and RNA FISH were used to detect the expression level and localization of hsa_circ_0001922 in PC-3 cells respectively. After hsa_circ_0001922 was targeted inhibited, clone formation, Transwell assay and scratch assay were used to detect the proliferation, migration and invasion abilities of PC-3 cells. qRT-PCR and Western blot were used to detect the expression levels of EMT pathway-related molecules after inhibiting hsa_circ_0001922.
ResultsThe expression of circular RNA hsa_circ_0001922 was increased in PC-3 cells (P < 0.01) and it existed in the cytoplasm and nucleus. The invasion, migration and invasion abilities were significantly weakened (P < 0.05) after inhibiting hsa_circ_0001922; the expression of E-cadherin mRNA increased (P < 0.05) while the mRNA level of Vimentin decreased (P < 0.05). The results of Western blot were consistent with the above (both P < 0.05).
ConclusionThe circular RNA hsa_circ_0001922 may promote the proliferation, migration and invasion of PC-3 cells through the EMT pathway.
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Key words:
- Prostate cancer /
- hsa_circ_0001922 /
- Proliferation /
- Invasion /
- EMT
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Competing interests: The authors declare that they have no competing interests.作者贡献：张妍妍：实验设计及论文撰写赵敏、刘静：细胞培养郭红燕、胡银英、赵林：课题思路指导王志刚：论文指导
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图 1 RNA FISH实验表明hsa-circ-0001922存在于前列腺癌PC-3细胞质和细胞核中
Figure 1 RNA FISH experiments showed that hsa_circ_0001922 existed in cytoplasm and nucleus of prostate cancer PC-3 cells
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图 2 不同浓度siRNA对hsa_circ_0001922表达的抑制效果
Figure 2 Inhibitory effect of different concentrations of siRNA on hsa_circ_0001922 expression
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图 3 敲低hsa_circ_0001922对前列腺癌PC-3细胞增殖(A)、侵袭(B)和迁移(C)的影响
Figure 3 Effect of hsa_circ_0001922 knockdown on proliferation(A), invasion(B) and migration(C) of prostate cancer PC-3 cells
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图 4 敲低hsa_circ_0001922对PC-3细胞EMT相关基因mRNA(A)和蛋白(B)表达的影响
Figure 4 Effects of hsa_circ_0001922 knockdown on mRNA(A) and protein(B) expression of EMT-related genes in PC-3 cells
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