The Experimental Study of Differentiation in the Rat C6 Gl ioma with all Trans Retinoic Acid
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摘要: 目的 探讨全反式维甲酸对大鼠C6脑胶质瘤的诱导分化效应及其机制。方法 建立大鼠C6脑胶质瘤模型，腹腔注射全反式维甲酸后，MRI检测胶质瘤的体积变化及流式细胞仪检测细胞增殖时相变化。结果 治疗组较对照组MRI检测显示肿瘤体积减小(P<0．05)，流式细胞仪检测显示G1期细胞比例增加(P<0．05)，S期细胞比例减少(P<0．05)。结论 全反式维甲酸可诱导C6胶质瘤分化．显著抑制胶质瘤细胞增殖，且其机制与改变细胞周期有关。Abstract: Objective To evaluate the effect and mechanism of the inducing differentiation of all t rans retinoic acid in the rat C6 glioma. Methods By establishing rat C6 glioma brain tumour model, af ter delivering all t rans retinoic acid via syringe injection into the peritoneal cavity, MRI and flow cytometer were employed to inspect tumour volume and cell cycle respectively. Results Tumour volume in the t reatment group was smaller than that in the cont rol group ( P < 0. 05), the ratio of the cells at G1 phase relatively increased ( P < 0. 05) and S phase relatively decreased ( P < 0. 05) in the t reatment group as compared with that of the cont rol group. Conclusion All t rans retinoic acid can induce differentiation and inhibit proliferation on rat C6 glioma, it s mechanism is concerned with the changes of the cell cycle.
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Key words:
- Glioma /
- All transretinoic acid /
- Inducing differentiation /
- MRI /
- Cell cycle
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