肾透明细胞癌中Cyclin F的表达及其对肾癌细胞株生物学行为的影响

苏敏; 王燕; 华结; 王天云; 徐胜男; 奎翔

doi:10.3971/j.issn.1000-8578.2025.24.0553

肾透明细胞癌中Cyclin F的表达及其对肾癌细胞株生物学行为的影响

Cyclin F Expression in Clear Cell Renal Cell Carcinoma and Its Effect on Biological Behavior of Renal Carcinoma Cell Lines

摘要

摘要:
目的探讨细胞周期蛋白F（Cyclin F）在肾透明细胞癌（ccRCC)中的表达、临床病理特征及其对肾癌细胞株生物学行为的影响。
方法 RT-qPCR和Western blot分别检测新鲜ccRCC标本中Cyclin F mRNA和蛋白表达。免疫组织化学法检测80例石蜡包埋样本中Cyclin F蛋白表达；CCK-8实验、划痕实验、流式细胞术检测过表达Cyclin F对肾癌细胞增殖、迁移和凋亡的影响。
结果 Cyclin F mRNA在癌组织中表达高于癌旁组织（P<0.0285），Cyclin F蛋白在癌组织中表达低于癌旁组织（P<0.001），临床病理参数分析显示，Cyclin F低表达与患者WHO/ISUP分级、Ki67指数和年龄显著相关（P=0.041，0.047，0.008,）。体外实验证实，过表达Cyclin F促进肾癌786-O细胞株的增殖（P<0.001）和迁移能力(P_{24 h}=0.003, P_{48 h}=0.0058)，抑制细胞凋亡（P=0.0019）。
结论 Cyclin F在ccRCC中蛋白低表达与患者高ISUP分级、高Ki67指数、高年龄及预后有关。

Abstract:
Objective To investigate the expression of Cyclin F in clear cell renal cell carcinoma (ccRCC), its clinicopathological characteristics, and its effect on the biological behavior of renal cancer cell lines
Methods RT-qPCR and Western blot were used to detect the mRNA and protein expression of Cyclin F in fresh ccRCC specimens. Immunohistochemistry assay was performed to detect the expression of Cyclin F protein in 80 paraffin samples. CCK-8 assay, scratch assay, and flow cytometry were conducted to determine the effects of Cyclin F overexpression on the proliferation, migration, and apoptosis of renal cancer cell lines.
Results The expression of Cyclin F in cancer tissues was higher than that in adjacent tissues at the mRNA level (P<0.0285). The expression of Cyclin Fprotein in cancer tissues was lower than that in adjacent tissues (P<0.001). The analysis of clinicopathological parameters showed that the low expression of Cyclin F was correlated with WHO/ISUP grade, Ki67 index, and age (P=0.041, 0.047, 0.008,). In vitro experiments confirmed that overexpression of Cyclin F promoted the proliferation (P<0.001) and migratory ability (24 h P=0.003, 48 h P=0.0058) of the RCC 786-O cell line, while inhibiting apoptosis (P=0.0019).
Conclusion The low expression of Cyclin F protein in ccRCC tissuesis associated with high ISUP grade, high Ki67 index, oldage, and prognosis of patients.

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