Abstract:
Objective Analysis of acid inositol-specific phospholipase-4gene mRNA expression and its clinical significance in hepatocellular carcinoma based on The Cancer Genome Atlas (TCGA) database.Methods Based on the data information of 424 clinical samples (including 374 cases of hepatocellular carcinoma tissues and 50 cases of non-tumour liver tissues) in the TCGA database, Kaplan-Meier method, Cox regression analysis, and immune infiltration analysis were used to evaluate the relationship between PLC4 gene and the clinical characteristics and survival prognosis of HCC patients. Correlation analysis between PLC4 gene and 24 types of immune cells was applied to investigate the relationship between PLC4gene and immune cell infiltration, and the correlation between the mRNA expression level of TP53 gene, a high-frequency mutation gene in hepatocellular carcinoma. In addition, paraffin sections of highly differentiated, moderately differentiated, and poorly differentiated tumour tissues as well as normal liver tissues from HCC patients in the Department of Pathology of the First Affiliated Hospital of Xinjiang Medical University were collected, each of which was 20 cases, and the histopathological observation was carried out by HE staining method, and the expression levels of PLC4 and Ki-67 proteins were verified by immunohistochemistry method in each clinical sample. Results The expression level of PLC4 gene in HCC was significantly higher than that in normal tissues (P < 0.01), and the overall survival of all patients in the PLC4 high-expression group was significantly longer than that in the low-expression group (P<0.05), suggesting that PLC4 significantly affects the prognosis of HCC patients. Correlation analysis showed that the expression level of PLC4 gene was highly correlated with immune cell infiltration; and PLC4 was highly correlated with the expression level of TP53 gene. As verified by clinical sample experiments, the results of HE staining experiments showed that immunohistochemistry results showed that PLC4 gene expression in HCC tissue samples was significantly higher than that in normal tissues (P<0.05), and it was positively correlated with the degree of differentiation.Conclusion PLC4 may serve as an independent prognostic factor in hepatocellular carcinoma and is expected to be a novel molecular target for HCC treatment.
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