Effects and Mechanism of LASP1 on Proliferation, Migration, and Apoptosis of Human Colorectal Cancer LOVO Cells
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摘要:目的
探讨LASP1基因表达对人结肠癌（LOVO）细胞增殖、迁移以及侵袭能力的影响及其作用机制。
方法分别构建LASP1过表达质粒和LASP1干扰质粒转染LOVO细胞，qRT-PCR验证LASP1 mRNA转染结果。MTT法、Tunel染色分别检测细胞增殖活性和细胞的凋亡情况，细胞划痕和Transwell实验检测细胞迁移和侵袭能力。Western blot测定细胞LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达。
结果质粒转染成功。LASP1过表达的LOVO细胞的增殖、迁移和侵袭能力增加，细胞凋亡率降低，细胞中LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达升高（P < 0.01）。而敲减LOVO细胞中LASP1的表达后，细胞增殖、迁移和侵袭能力减弱，细胞凋亡率增加，细胞中LASP1、p-FAK/FAK、p-AKT/AKT蛋白表达降低（P < 0.01）。
结论LASP1可正向调控FAK/AKT信号通路，促进LOVO细胞的增殖、迁移以及侵袭能力。
Abstract:ObjectiveTo explore the effects and mechanism of LASP1 gene expression on the proliferation, migration, and invasion of human colorectal cancer (LOVO) cells.
MethodsLASP1 overexpression plasmids and LASP1 interference plasmids were constructed and transfected to LOVO cells. qRT-PCR was used to detect LASP1 mRNA expression and validate the transfection. MTT method and Tunel staining were used to detect cell proliferation and apoptosis, respectively, and scratch test and Transwell test were employed to determine the migration and invasion abilities of cells. Western blot was applied to analyze the expression of LASP1, p-FAK/FAK, and p-AKT/AKT protein in cells.
ResultsThe plasmids were successfully transfected. LASP1 overexpression increased the proliferation, migration, and invasion of LOVO cells, decreased the apoptosis, and increased LASP1, p-FAK/FAK, p-AKT/AKT protein expression (P < 0.01). LASP1 knockdown reduced the proliferation, migration, and invasion of LOVO cells, increased the apoptosis, and decreased LASP1, p-FAK/FAK, and p-AKT/AKT protein expression (P < 0.01).
ConclusionLASP1 positively regulates the FAK/AKT signaling pathway to promote the proliferation, migration, and invasion of LOVO cells.
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Key words:
- Colorectal cancer /
- LOVO cells /
- LASP1 gene /
- Metastasis /
- Invasion /
- FAK/AKT signaling pathway
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Competing interests: The authors declare that they have no competing interests.利益冲突声明：所有作者均声明不存在利益冲突。作者贡献：徐益平：数据分析及文章撰写与修改尚韬：实验思路构思及指导
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图 1 LASP1过表达或沉默对LOVO细胞迁移及侵袭能力的影响
Figure 1 Effects of LASP1 overexpression or silence on migration and invasion of LOVO cells
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图 2 LASP1过表达或沉默对LOVO细胞凋亡的影响(Tunel染色)
Figure 2 Effects of LASP1 overexpression or silence on apoptosis of LOVO Cells (Tunel staining)
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图 3 LASP1过表达或沉默对LOVO细胞相关蛋白表达的影响
Figure 3 Effects of LASP1 overexpression or silence on related protein expression in LOVO cells
表 2 qRT-PCR检测LOVO细胞转染后LASP1的表达
Table 2 LASP1 mRNA expression in transfected LOVO cells detected by qRT-PCR
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表 3 LASP1过表达或沉默对LOVO细胞存活的影响
Table 3 Effects of overexpression or silencing of LASP1 on the survival of LOVO cells
下载: 导出CSV
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