Research Progress of Single-cell Transcriptome Sequencing in Immune Microenvironment of Nasopharyngeal Carcinoma
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摘要:
分析近年来鼻咽癌单细胞转录组测序的研究进展，对鼻咽癌免疫微环境进行剖析，总结髓系细胞、T细胞、B细胞、NK细胞等免疫细胞和成纤维细胞在鼻咽癌组织中的作用机制，为鼻咽癌的诊断、治疗和预后预测提供参考。
Abstract:We analyze the recent research progress of single-cell transcriptome sequencing of nasopharyngeal carcinoma (NPC), dissect its tumor immune microenvironment, and focus on the role of myeloid cells, T cells, B cells, NK cells, and fibroblasts in the mechanism of action in NPC tissues to provide reference for the diagnosis, treatment, and prognosis prediction of NPC.
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Competing interests: The authors declare that they have no competing interests.作者贡献：应敏：文献整理、论文构思及撰写刘金坤：论文指导、校对及修改
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图 1 鼻咽癌免疫微环境中细胞募集和细胞间相互作用的示意图
Figure 1 Schematic illustration of cell recruitment and cell–cell interactions in the nasopharyngeal carcinoma immune microenvironment
Immune cells infiltrate into the tumor tissue from blood vessels under the effect of chemokines, thereby interacting with tumor cells.
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[1] Sung H, Ferlay J, Siegel RL, et al. Global Cancer Statistics 2020: GLOBOCAN Estimates of Incidence and Mortality Worldwide for 36 Cancers in 185 Countries[J]. CA Cancer J Clin, 2021, 71(3): 209-249. doi: 10.3322/caac.21660
[2] Zhu Q, Zhao G, Li Y, et al. Advances in pathogenesis and precision medicine for nasopharyngeal carcinoma[J]. MedComm, 2021, 2(2): 175-206. doi: 10.1002/mco2.32
[3] Wang YQ, Chen YP, Zhang Y, et al. Prognostic significance of tumor-infiltrating lymphocytes in nondisseminated nasopharyngeal carcinoma: A large-scale cohort study[J]. Int J Cancer, 2018, 142(12): 2558-2566. doi: 10.1002/ijc.31279
[4] Lei Y, Tang R, Xu J, et al. Applications of single-cell sequencing in cancer research: progress and perspectives[J]. J Hematol Oncol, 2021, 14(1): 1-26. doi: 10.1186/s13045-020-01025-7
[5] Luecken MD, Theis FJ. Current best practices in single-cell RNA-seq analysis: a tutorial[J]. Mol Syst Biol, 2019, 15(6).
[6] Ren X, Zhang L, Zhang Y, et al. Insights Gained from Single-Cell Analysis of Immune Cells in the Tumor Microenvironment[J]. Annu Rev Immunol, 2021, 39: 583-609. doi: 10.1146/annurev-immunol-110519-071134
[7] Chen YP, Yin JH, Li WF, et al. Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma[J]. Cell Res, 2020, 30(11): 1024-1042. doi: 10.1038/s41422-020-0374-x
[8] Zhang L, Li Z, Skrzypczynska KM, et al. Single-Cell Analyses Inform Mechanisms of Myeloid-Targeted Therapies in Colon Cancer[J]. Cell, 2020, 181(2): 442-459. doi: 10.1016/j.cell.2020.03.048
[9] Gong L, Kwong DLW, Dai W, et al. Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma[J]. Nat Commun, 2021, 12(1): 1-18. doi: 10.1038/s41467-020-20314-w
[10] Jin S, Li R, Chen MY, et al. Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma[J]. Cell Res, 2020, 30(11): 950-965. doi: 10.1038/s41422-020-00402-8
[11] Cheng S, Li Z, Gao R, et al. A pan-cancer single-cell transcriptional atlas of tumor infiltrating myeloid cells[J]. Cell, 2021, 184(3): 792-809. doi: 10.1016/j.cell.2021.01.010
[12] Zhang Q, He Y, Luo N, et al. Landscape and Dynamics of Single Immune Cells in Hepatocellular Carcinoma[J]. Cell, 2019, 179(4): 829-845. doi: 10.1016/j.cell.2019.10.003
[13] Michea P, Noël F, Zakine E, et al. Adjustment of dendritic cells to the breast-cancer microenvironment is subset specific[J]. Nat Immunol, 2018, 19(8): 885-897. doi: 10.1038/s41590-018-0145-8
[14] Zilionis R, Engblom C, Pfirschke C, et al. Single-Cell Transcriptomics of Human and Mouse Lung Cancers Reveals Conserved Myeloid Populations across Individuals and Species[J]. Immunity, 2019, 50(5): 1317-1334. doi: 10.1016/j.immuni.2019.03.009
[15] Liu Y, Zhang Q, Xing B, et al. Immune phenotypic linkage between colorectal cancer and liver metastasis[J]. Cancer Cell, 2022, 40(4): 424-437. doi: 10.1016/j.ccell.2022.02.013
[16] Podolsky MA, Bailey JT, Gunderson AJ, et al. Differentiated state of initiating tumor cells is key to distinctive immune responses seen in H-RasG12V-induced squamous tumors[J]. Cancer Immunol Res, 2017, 5(3): 198-210. doi: 10.1158/2326-6066.CIR-16-0304
[17] Ren XW, Zhang L, Zhang YY, et al. Insights Gained from Single-Cell Analysis of Immune Cells in the Tumor Microenvironment[J]. Annu Rev Immunol, 2021, 39: 583-609. doi: 10.1146/annurev-immunol-110519-071134
[18] Mizukami Y, Kono K, Kawaguchi Y, et al. CCL17 and CCL22 chemokines within tumor microenvironment are related to accumulation of Foxp3+ regulatory T cells in gastric cancer[J]. Int J Cancer, 2008, 122(10): 2286-2293. doi: 10.1002/ijc.23392
[19] Wu H, Gong J, Liu Y. Indoleamine 2, 3-dioxygenase regulation of immune response[J]. Mol Med Rep, 2018, 17(4): 4867-4873.
[20] Jang JE, Hajdu CH, Liot C, et al. Crosstalk between Regulatory T Cells and Tumor-Associated Dendritic Cells Negates Anti-tumor Immunity in Pancreatic Cancer[J]. Cell Rep, 2017, 20(3): 558-571. doi: 10.1016/j.celrep.2017.06.062
[21] Sánchez-Paulete AR, Teijeira A, Cueto FJ, et al. Antigen cross-presentation and T-cell cross-priming in cancer immunology and immunotherapy[J]. Ann Oncol, 2017, 28: 74.
[22] Liu Y, He S, Wang XL, et al. Tumour heterogeneity and intercellular networks of nasopharyngeal carcinoma at single cell resolution[J]. Nat Commun, 2021, 12(1): 1-18. doi: 10.1038/s41467-020-20314-w
[23] Peng WS, Zhou X, Yan WB, et al. Dissecting the heterogeneity of the microenvironment in primary and recurrent nasopharyngeal carcinomas using single-cell RNA sequencing[J]. Oncoimmunology, 2022, 11(1): 2026583. doi: 10.1080/2162402X.2022.2026583
[24] Li JP, Wu CY, Chen MY, et al. PD-1+CXCR5-CD4+Th-CXCL13 cell subset drives B cells into tertiary lymphoid structures of nasopharyngeal carcinoma[J]. J Immunother Cancer, 2021, 9(7): e002101. doi: 10.1136/jitc-2020-002101
[25] Gong L, Kwong DL, Dai W, et al. Comprehensive single-cell sequencing reveals the stromal dynamics and tumor-specific characteristics in the microenvironment of nasopharyngeal carcinoma[J]. Nat Commun, 2021, 12(1): 1-18. doi: 10.1038/s41467-020-20314-w
[26] Workel HH, Lubbers JM, Arnold R, et al. A Transcriptionally Distinct CXCL13+CD103+CD8+ T-cell Population Is Associated with B-cell Recruitment and Neoantigen Load in Human Cancer[J]. Cancer Immunol Res, 2019, 7(5): 784-796. doi: 10.1158/2326-6066.CIR-18-0517
[27] Zhang Y, Chen H, Mo H, et al. Single-cell analyses reveal key immune cell subsets associated with response to PD-L1 blockade in triple-negative breast cancer[J]. Cancer Cell, 2021, 39(12): 1578-1593. doi: 10.1016/j.ccell.2021.09.010
[28] He J, Xiong X, Yang H, et al. Defined tumor antigen-specific T cells potentiate personalized TCR-T cell therapy and prediction of immunotherapy response[J]. Cell Res, 2022, 32(6): 530-542. doi: 10.1038/s41422-022-00627-9
[29] Jin S, Li R, Chen MY, et al. Single-cell transcriptomic analysis defines the interplay between tumor cells, viral infection, and the microenvironment in nasopharyngeal carcinoma[J]. Cell Res, 2020, 30(11): 950-965. doi: 10.1038/s41422-020-00402-8
[30] Thommen DS, Koelzer VH, Herzig P, et al. A transcriptionally and functionally distinct pd-1+ cd8+ t cell pool with predictive potential in non-small-cell lung cancer treated with pd-1 blockade[J]. Nat Med, 2018, 24(7): 994-1004. doi: 10.1038/s41591-018-0057-z
[31] De Groen RA, Groothuismink ZM, Liu BS, et al. IFN-is able to augment TLR-mediated activation and subsequent function of primary human B cells[J]. J Leukoc Biol, 2015, 98(4): 623-630. doi: 10.1189/jlb.3A0215-041RR
[32] Hervas-Stubbs S, Perez-Gracia JL, Rouzaut A, et al. Direct effects of type I interferons on cells of the immune system[J]. Clin Cancer Res, 2011, 17(9): 2619-2627. doi: 10.1158/1078-0432.CCR-10-1114
[33] Kyriakidis NC, Kapsogeorgou EK, Gourzi VC, et al. Toll-like receptor 3 stimulation promotes Ro52/TRIM21 synthesis and nuclear redistribution in salivary gland epithelial cells, partially via type I interferon pathway[J]. Clin Exp Immunol, 2014, 178(3): 548-560. doi: 10.1111/cei.12432
[34] Böttcher JP, Bonavita E, Chakravarty P, et al. NK Cells Stimulate Recruitment of cDC1 into the Tumor Microenvironment Promoting Cancer Immune Control[J]. Cell, 2018, 172(5): 1022-1037. doi: 10.1016/j.cell.2018.01.004
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