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摘要:目的
探讨大气细颗粒物（particulate matter，PM）PM2.5致肺部病变机制。
方法采用实时PM2.5采集器进行PM2.5收集，Wistar大鼠饲养于PM2.5的全身暴露染毒系统，Wistar大鼠每日染毒4小时，共染毒9月，设立实验组及对照组；观察肺组织大体结构及HE染色观察；流式细胞术检测Wistar大鼠T细胞免疫功能、肺组织细胞凋亡、细胞周期及DNA指数；ELISA实验检测Wistar大鼠血清及肺灌洗液中CEA、CA125及SccAg表达水平。
结果实验组肺组织以支气管向外周辐射状颜色发暗、偏黑，多处可见白色斑点和泡状凸起，边缘不规则白色隆起；HE染色结果，实验组肺组织纤维化，肺实变严重，肺泡充血，见大量炎性反应增生；实验组CD4/CD8比值显著高于对照组（P < 0.05），血清及肺灌洗液中CA125的表达显著高于对照组（P < 0.05），实验组肺组织细胞凋亡率显著低于对照组，而DNA指数显著增高（均P < 0.05）。
结论长期接触PM2.5会造成肺组织出现不同程度结节，肺组织出现大范围炎性反应病变。
Abstract:ObjectiveTo investigate the mechanism of lung lesions caused by fine particulate matter PM2.5.
MethodsReal-time PM2.5 collector was used to collect PM2.5. Wistar rats were fed on the systemic exposure system of PM2.5. Wistar rats were exposed to 4 hours daily for 9 months. PM2.5 experimental group and control group were set up. General structure of lung tissue and HE staining result were observed. T cell immune function, lung cell apoptosis, cell cycle and DNA index in Wistar rats were detected by flow cytometry. The expression levels of CEA, CA125 and SccAg in serum and lung lavage fluid of Wistar rats were detected by ELISA.
ResultsLung tissues of experimental group were radiated from the periphery of the bronchus with dark, visible white spots and bubble like protrusions with irregular white ridges. HE staining results showed that lung tissues of experimental group were fibrotic, severe consolidation of lung, alveolar hyperemia and massive inflammaroty reactive hyperplasia. CD4/CD8 ratio in experimental group was significantly higher than that in control group(P < 0.05). The expression of CA125 in serum and lung lavage fluid was significantly higher than that in control group(P < 0.05). The apoptosis rate of lung tissues in experimental group was significantly lower than that in control group, but DNA index was significantly higher (P < 0.05).
ConclusionLong-term exposure to PM2.5 results in different degrees of nodules and a large range of inflammatory reaction in lung tissues.
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Key words:
- Fine particulate matter /
- Smog /
- Pulmonary lesions /
- DNA index /
- Cell proliferation
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图 6 流式细胞术检测Wistar大鼠静脉血CD3、CD4、CD8表达水平
Figure 6 Expression of CD3, CD4 and CD8 in venous blood of Wistar rats detected by flow cytometry
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图 7 ELISA实验检测Wistar大鼠血清及肺灌洗液中CEA、CA125及SccAg表达水平
Figure 7 Expression levels of CEA, CA125 and SccAg in serum and lung lavage fluid of Wistar rats detected by ELISA assay
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图 8 流式细胞术检测Wistar大鼠肺组织细胞周期
Figure 8 Cell cycle of Wistar rats lung tissues detected by flow cytometry
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图 9 流式细胞术检测Wistar大鼠肺组织细胞凋亡
Figure 9 Cell apoptosis of Wistar rats lung tissue detected by flow cytometry
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[1] 曾其莉, 岳苗苗, 谢曙光, 等.华中某市居民住宅室内外PM2.5与PM10日变化规律及防护建议[J].公共卫生与预防医学, 2015, 26(2):40-2. http://www.cnki.com.cn/Article/CJFDTOTAL-FBYF201502012.htm Zeng QL, Yue MM, Xie SG, et al. Daily change patterns of indoor and outdoor PM2.5 and PM10 concentration in Central China city residences and protection suggestions[J]. Gong Gong Wei Sheng Yu Yu Fang Yi Xue, 2015, 26(2):40-2. http://www.cnki.com.cn/Article/CJFDTOTAL-FBYF201502012.htm
[2] Puett RC, Hart JE, Yanosky JD, et al. Particulate matter air pollution exposure, distance to road, and incident lung cancer in the nurses' health study cohort[J]. Environ Health Perspect, 2014, 122(9):926-32. https://ehp.niehs.nih.gov/1307490/
[3] Atkinson RW, Carey IM, Kent AJ, et al. Long-term exposure to outdoor air pollution and incidence of cardiovascular diseases[J]. Epidemiology, 2013, 24(1):44-53. doi: 10.1097/EDE.0b013e318276ccb8
[4] 李玉洁, 陈颖, 王娅杰, 等. PM2.5暴露诱发动脉粥样硬化斑块失稳定发病机制及应对策略思考[J].中国中药杂志, 2014, 39(15):2978-82. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201415038.htm Li YJ, Chen Y, Wang YJ, et al. Pathogenesis of plaque destabilization induced by PM2.5 exposure and coping strategies[J]. Zhongguo Zhong Yao Za Zhi, 2014, 39(15):2978-82. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGZY201415038.htm
[5] 陶永进, 曾娅莉, 邓建军, 等. PM2.5沙尘矿物的6中主要成分致A549细胞遗传毒性研究[J].工业卫生与职业病, 2016, 42(5):321-5. http://www.cnki.com.cn/Article/CJFDTOTAL-GYWZ201605001.htm Tao YJ, Zeng YL, Deng JJ, et al. Study on genotoxicity of six main kinds of PM2.5 mineral particles in A549 cell[J]. Gong Ye Wei Sheng Yu Zhi Ye Bing, 2016, 42(5):321-5. http://www.cnki.com.cn/Article/CJFDTOTAL-GYWZ201605001.htm
[6] Mahalingaiah S, Hart JE, Laden F, et al. Air pollution and risk of uterine leiomyomata[J]. Epidemiology, 2014, 25(5):682-8. doi: 10.1097/EDE.0000000000000126
[7] Mahalingaiah S, Hart JE, Laden F, et al. Air pollution exposures during adulthood and risk of endometriosis in the Nurses' Health Study Ⅱ[J]. Environ Health Perspect, 2014, 122(1):58-64. https://ehp.niehs.nih.gov/1306627/
[8] Hart JE, Källberg H, Laden F, et al. Ambient air pollution exposures and risk of rheumatoid arthritis[J]. Arthritis Care Res (Hoboken), 2013, 65(7):1190-6. doi: 10.1002/acr.21975
[9] 刘莎莎, 敖登其木格, 王红丽, 等. PM2.5通过活化NF-κB途径诱导支气管上皮细胞中血管内皮生长因子的表达[J].军事医学, 2015, 39(5):325-8. http://www.cnki.com.cn/Article/CJFDTOTAL-JSYX201505003.htm Liu SS, Aodengqimuge, Wang HL, et al. PM2.5 induces VEGF expression via activation of NF-κB pathway in bronchial epithelial cells[J]. Jun Shi Yi Xue, 2015, 39(5):325-8. http://www.cnki.com.cn/Article/CJFDTOTAL-JSYX201505003.htm
[10] 肖纯凌, 李舒音, 尚德志, 等. PM2.5大气污染物致大鼠呼吸系统病理学变化[J].中国公共卫生, 2011, 27(12):1579-81. doi: 10.11847/zgggws2011-27-12-33 XIAO Chun-ling, LI Shu-yin, SHANG De-zhi, et al. Pathologic changes in trachea of rats exposed to PM2.5 artificial air pollution[J]. Zhongguo Gong Gong Wei Sheng, 2011, 27(12):1579-81. doi: 10.11847/zgggws2011-27-12-33
[11] 杨凌, 马琼锦, 李莉珊, 等. PM2.5亚慢性染毒对小鼠肺部炎症及Th17/Treg的影响[J].卫生研究, 2014, 43(3):387-92. http://www.cnki.com.cn/Article/CJFDTOTAL-WSYJ201403008.htm Yang L, Ma QG, Li LS, et al. Effects of airborne fine particulate matters on pulmonary inflammation injury and Th17/Treg balance of sub-chronic exposure mice[J]. Wei Sheng Yan Jiu, 2014, 43(3):387-92. http://www.cnki.com.cn/Article/CJFDTOTAL-WSYJ201403008.htm
[12] 邓元荣, 李泳宁, 黄晓敏, 等.大气细颗粒物吸入动物模型建立及适用性评价[J].中国比较医学杂志, 2016, 26(9):42-9. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX201609010.htm Deng YR, Li YN, Huang XM, et al. Establishment and evaluation of animal model induced by inhalation injury of airborne fine particulate matter[J]. Zhongguo Bi Jiao Yi Xue Za Zhi, 2016, 26(9):42-9. http://www.cnki.com.cn/Article/CJFDTOTAL-ZGDX201609010.htm
[13] 周莉婷, 杨果, 古桂雄, 等.苏州市独墅湖高教区PM2.5对大鼠呼吸系统急性损伤的研究[J].环境卫生学杂志, 2016, 6(2):97-101. http://www.cnki.com.cn/Article/CJFDTOTAL-GWYX201602002.htm Zhou LT, Yang G, Gu GX, et al. Acute Injury of Respiratory System in Rats Induced by PM2.5 Collected from Dushu Lake Higher Education Town in Suzhou[J]. Huan Jing Wei Sheng Xue Za Zhi, 2016, 6(2):97-101. http://www.cnki.com.cn/Article/CJFDTOTAL-GWYX201602002.htm
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