Expression of Cytoskeletal Protein 4.1N, E-cadherin and β-catenin in Gastric Carcinoma and Their Clinical Significance
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摘要: 目的 探讨胃癌组织、癌旁胃组织中细胞骨架蛋白4.1N与E-cadherin、β-catenin在胃癌转移扩散中的作用及三者间的相互关系。方法 收集52例胃癌标本（腺癌）、30例距癌组织边缘10 cm以上的癌旁胃组织标本作为对照。应用免疫组织化学Elivision法检测这些组织中的细胞骨架蛋白4.1N与E-cadherin、β-catenin的蛋白表达情况。结果 胃癌组织中细胞骨架蛋白4.1N、E-cadherin及β-catenin 蛋白表达明显低于对照组（P<0.05）；低分化胃癌组织中细胞骨架蛋白4.1N与E-cadherin、β-catenin的蛋白表达较高-中分化胃癌组织表达显著降低（P<0.05）；细胞骨架蛋白4.1N与E-cadherin、β-catenin 的蛋白在无淋巴结转移的胃癌组与有淋巴结转移的胃癌组间比较差异有统计学意义（P<0.05）；细胞骨架蛋白4.1N与E-cadherin、β-catenin的蛋白在胃癌组织中的IOD值随着TNM分期的增高而降低，组间比较差异具有统计学意义（P<0.05），细胞骨架蛋白4.1N与E-cadherin、β-catenin的蛋白的表达与胃癌患者的年龄、性别无关（P>0.05）；细胞骨架蛋白4.1N与E-cadherin（r=0.381，P<0.05）、E-cadherin 与β-catenin（r=0.571，P<0.05）、细胞骨架蛋白4.1N与β-catenin（r=0.602，P<0.05）之间均呈正相关关系。结论 细胞骨架蛋白4.1N与E-cadherin、β-catenin在胃癌中的表达呈正相关，三者在胃癌发生发展中可能具有重要的协同作用。
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关键词:
- 胃癌 /
- 细胞骨架蛋白4.1N /
- E-钙黏着素 /
- β-连环蛋白 /
- 免疫组织化学
Abstract: Objective To explore the significance and interrelationship the protein expression of cyftoskeletal protein 4.1N, E-cadherin and β-catenin in the metastatic proliferation of gastric cancer. Methods We collected 55 cases of gastric carcinoma (adenocarcinoma) and 30 cases of gastric mucosa (distance from tumor edge ≥10 cm) as control study removed by surgical operation. The expressions of cytoskeletal protein 4.1N,E-cadherin and β-catenin protein were detected by Elivision immunohistochemicaly assay. Results The protein expression of cytoskeletal protein 4.1N, E-cadherin and β-catenin in gastric cancer group were significantly lower than that in control group (P<0.05), moreover, that in poorly-differentiated group was significantly lower than those in well- and moderately-differentiated groups (P<0.05). The protein expression of these three proteins between gastric cancer with and without lymph node metastasis group had significant difference (P<0.05). The IOD values of these three proteins were decreased with the increase of TNM stages in gastric cancer group. Similarly there was significant difference between the two groups (P<0.05). The expression of these proteins were not related with sex or age (P>0.05). There were positive correlation between cytoskeleton protein 4.1N and E-cadherin protein (r=0.381, P<0.05), E-cadherin protein and β-catenin (r=0.571, P<0.05), cytoskeleton protein 4.1N and β-catenin(r=0.602, P<0.05). Conclusion There were positive correlations among the expressions of cytoskeletal protein 4.1N, E-cadherin and β-catenin, indicating that important synergies exist among three proteins in promoting occurrence and development of gastric cancer.-
Key words:
- Gastric cancer /
- Ctoskeletal protein 4.1N /
- E-cadherin /
- β-catenin /
- Imunohistochemis
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