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羧基末端结合蛋白1对肝癌细胞HepG2增殖的影响

李运千, 黄承信, 曾思恩, 刘晓佳, 刘 勤, 肖胜军

李运千, 黄承信, 曾思恩, 刘晓佳, 刘 勤, 肖胜军. 羧基末端结合蛋白1对肝癌细胞HepG2增殖的影响[J]. 肿瘤防治研究, 2014, 41(05): 384-387. DOI: 10.3971/j.issn.1000-8578.2014.05.008
引用本文: 李运千, 黄承信, 曾思恩, 刘晓佳, 刘 勤, 肖胜军. 羧基末端结合蛋白1对肝癌细胞HepG2增殖的影响[J]. 肿瘤防治研究, 2014, 41(05): 384-387. DOI: 10.3971/j.issn.1000-8578.2014.05.008
LI Yunqian, HUANG Chenxin, ZENG Sien, LIU Xiaojia, LIU Qin, XIAO Shengjun. Impact of C-terminal Binding Protein 1 on Cell Proliferation of Hepatocellular Carcinoma Cell HepG2[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 384-387. DOI: 10.3971/j.issn.1000-8578.2014.05.008
Citation: LI Yunqian, HUANG Chenxin, ZENG Sien, LIU Xiaojia, LIU Qin, XIAO Shengjun. Impact of C-terminal Binding Protein 1 on Cell Proliferation of Hepatocellular Carcinoma Cell HepG2[J]. Cancer Research on Prevention and Treatment, 2014, 41(05): 384-387. DOI: 10.3971/j.issn.1000-8578.2014.05.008

羧基末端结合蛋白1对肝癌细胞HepG2增殖的影响

详细信息
    作者简介:

    李运千(1974-),男,硕士,主治医师,主要从事肿瘤发生分子机制的基础和临床研究

    通信作者:

    肖胜军,E-mail:kent805xsj@gmail.com

  • 中图分类号: R735.7

Impact of C-terminal Binding Protein 1 on Cell Proliferation of Hepatocellular Carcinoma Cell HepG2

  • 摘要: 目的 研究羧基末端结合蛋白1(C-terminal binding protein1,CtBP1)对人肝癌细胞HepG2增殖的影响及机制。 方法 采用CtBP1 siRNA转染HepG2细胞,实验分为4个组:空白对照组、转染试剂对照组、阴性序列对照组与干扰组。于转染后不同的时间收集细胞,用MTT法检测细胞增殖、流式细胞术检测细胞周期及凋亡情况。结果 CtBP1 siRNA转染有效抑制了HepG2细胞中CtBP1蛋白表达,与空白对照组比较,转染后72 h后,CtBP1蛋白下调了57.80%。 CtBP1沉默后细胞生长受到抑制(P<0.05),转染后24、48、72、96 h生长抑制率分别为22.34%、52.15%、53.97%和54.77%;而对细胞周期分布无明显影响(P>0.05);但CtBP1表达下调有促凋亡作用,与对照组相比,干扰组的细胞凋亡率显著升高(P<0.05)。结论 CtBP1沉默能抑制HepG2细胞增殖,其机制是通过促凋亡来实现的。CtBP1能促进肿瘤生长和抑制凋亡,是潜在的肿瘤治疗靶点。

     

    Abstract: Obiective To investigate the impaction of C-terminal binding protein 1 (CtBP1) on proliferation of hepatocellular carcinoma cell HepG2 and related mechanism. Methods siRNA targeting for CtBP1 were introduced into HepG2 cells to knock down CtBP1,3 control groups including blank control, transfection reagent and control siRNA were used as controls.At different time points after transfection, HepG2 cells were collected for MMT assay to detect cell proliferation and fl owcytometry to detect cell cycle distribution and apoptosis. Results CtBP1 siRNA effectively knocked down CtBP1 in HepG2 cell. Compared with control group, CtBP1 protein signifi cantly was decreased by 57.80%.Cell growth were signifi cantly inhibited after CtBP1 knockdown (P<0.05). The inhibition rates of cell growth were 22.34%,52.15%,53.97% and 54.77% at 24, 48, 72 and 96 h respectively. The distribution of cell cycle was not changed but apopotic cells were increased significantly compared with control group. Conclusion CtBP1 knockdown could inhibit cell proliferation of HepG2 mainly through apoptosis instead of cell cycle arrest. CtBP1 is involved in cell growth and apoptosis inhibition of tumor cells, and could be a candidate for cancer targeted therapy.

     

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出版历程
  • 收稿日期:  2013-04-28
  • 修回日期:  2013-07-01
  • 刊出日期:  2014-05-24

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