5-氮杂-2′-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响

刘瑶; 贺兴波; 谢军; 孟凡; 杨建琼; 黄才斌

doi:10.3971/j.issn.1000-8578.2012.01.003

5-氮杂-2′-脱氧胞苷对肝癌细胞HepG2凋亡及其PEG10基因表达的影响

1.341000江西赣州，赣南医学院第一附属医院消化内科,2.医务科,3.临床医学研究中心

详细信息

中图分类号: R735.7
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出版历程
- 收稿日期: 2011-05-30
- 修回日期: 2011-09-07
- 刊出日期: 2012-01-24

Effects of 5-Aza-2′-deoxycytidine on Apoptosis and Expression of PEG10 Gene in Human Hepato Carcinoma Cell Line HepG2

1.Department of Gastroenterology，The First Affiliated Hospital of Gannan Medical University，Ganzhou 341000，China,2.Department of Medical Services，3.Clinical Medical Research Center

摘要

摘要: 目的探讨去甲基化药物5-氮杂-2′-脱氧胞苷（5-Aza-CdR）对人肝癌细胞系HepG2凋亡及其PEG10基因表达调控的影响，进一步探讨肝癌的发病机制及5-Aza-CdR治疗肝癌的可行性。方法用浓度为50 μmol/L的5-Aza-CdR分别处理HepG2细胞24、48、72 h，hoechst 33342/PI 双染荧光显微镜检测HepG2细胞凋亡情况。RT-PCR、Western blot法分别检测PEG10 mRNA和蛋白表达水平的变化。结果与对照组相比，5-Aza-CdR作用24、48、72 h后，HepG2细胞的凋亡率明显升高，PEG10 mRNA 和蛋白表达水平显著下降，并呈现时间依赖性（P＜0.05）。结论5-Aza-CdR 可促进HepG2细胞凋亡并抑制PEG10基因的表达。
- 5-氮杂-2′-脱氧胞苷 /
- PEG10 /
- 凋亡 /
- HepG2细胞
Abstract: Objective To investigate the effects of demethyhfing agent 5′-Aza-2′-deoxyeytidine(5-Aza-CdR)on the expression of PEG10 gene in human hepato carcinoma cell line HepG2. Methods HepG2 cells were treated with 50.0 μmol/L 5-Aza-CdR for 24,48,72 h.Apoptosis of HepG2 induced by 5-Aza-CdR was identified by fluorescence microscopy with hoechst 33342/PI staining.RT-PCR and Western blot were applied to detect the PEG10 mRNA and protein expression levels,respectively. Results After treated with 5-Aza-CdRat for indicated time,fluorescence microscopy analysis showed that the apoptosis rate of HepG2 cells increased significantly,and the expression of PEG10 mRNA and protein in HepG2 cells were reduced in a time-dependent manner(P＜0.05). Conclusion 5-Aza-CdR can induce HepG2 cell apoptosis and reduce the expression of PEG10 gene.
- 5-Aza-CdR /
- PEG10 /
- Apoptosis /
- HepG2 cell