Objective To investigate the effect of annexin A5 knockdown on the proliferation and apoptosis of gastric cancer MKN-45 cells.
Methods The gastric cancer MKN-45 cells were divided into three groups: siRNA interference group transfected with siRNA targeting to ANXA5, negative control group transfected with scrambled siRNA and blank group without any treatment. After 48h of transfection, qRT-PCR and Western blot were used to detect the suppression of ANXA5 on both mRNA and protein levels. MTT and clone formation assays were used to detect the effect of low annexin A5 expression on the proliferation of gastric cancer MKN-45 cells. Cell apoptosis was analyzed by flow cytometry. Western blot was performed to detect the expression of PCNA and Cyclin D1.
Results After transfection with siRNA targeting to annexin A5, the expression of annexin A5 in gastric cancer MKN-45 cells was significantly lower than those in the negative and blank control groups (P < 0.05). The results of MTT and clone formulation assays showed that cell proliferation activity and colony-forming ability of siRNA interference group were significantly higher than those of negative and blank control groups (P < 0.05), whereas cell apoptosis was not affected statistically (P > 0.05). The expression of PCNA and Cyclin D1 were significantly increased.
Conclusion Low annexin A5 expression could promote the proliferation of gastric cancer cells.
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