Abstract: Objective To observe the biological characteristics of CIK cells activated by recombinant human fibronectin(RN) and establish an effective way of cell proliferation. Methods We separated the mononuclear cells(PBMCs) from 50 ml peripheral blood from 15 HCC patients PBMCs were divided into two groups,of which were activated by RN-induced (RN+anti-CD3 mAb+IL-2) or conventional method(anti-CD3 mAb+IFN-γ+IL-2) repectively.Then we estimated cell morphology,the number,cell phenotype and cytotoxic efficiency at different time points in two groups. Results RN-induced group had a 2.0~2.8 fold higher proliferation rate,compared with conventional group,P<0.05.There was also a higher percentage of activated T cells and CD3⁺CD8⁺T cells in RN-induced group.We observed a higher inhibition rate of the CIK cells to liver cancer cell lines. Conclusion RN may replace conventional method to induce CIK cell proliferation in HCC patients.