microRNA-31在原发性肺癌组织中的表达及临床意义
Expression and Clinical Significance of microRNA-31 in Primary Lung Cancer Tumor Tissues
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摘要: 目的 检测肺癌组织及癌旁正常组织中miR-31的表达情况与肺癌的诊断、肿块大小、淋巴结转移、吸烟及分期的关系。方法 采用quantitative real-time RT-PCR方法对75例肺癌患者术后癌组织及癌旁正常组织中miR-31的表达量进行检测。结果 miR-31在癌组织中的相对表达量是癌旁正常组织的6.48倍(P<0.001)。吸烟患者miR-31的表达量是不吸烟患者的3.06倍(P=0.033)。在NSCLC中，与肿瘤长径≤3 cm肿瘤相比，长径＞3 cm肿瘤中miR-31的表达量较高(P=0.045)。无论淋巴结转移与否，其表达量差异无统计学意义(P=0.703)。结论 miR-31在肺癌组织中高表达、在不同肿块中表达的差异以及与吸烟的关系，提示其可能成为肺癌的筛查、诊断及预测肿块大小的一个生物标志物。Abstract: Objective Detected the miR-31 expresssion level in tumor tissues and adjacent normal tissues to observe the relationship between miR-31 and the screening & diagnosis of lung cancer. Methods Using quantitative real-time RT-PCR to assess the miR-31 expression in lung cancer tumor tissues and adjacent normal tissues. Results The relative expression level of miR-31 is 6.48 folds in tumor tissues compared to adjacent normal tissues（P<0.001).The level in smoke patients specimen is 3.06 folds to non-smoke.The expression level of tumor major axis＞3 cm is higher than major axis≤3 cm in NSCLC (P=0.033).Whether the lymph nodes metastasis or not，there is no difference between them. Conclusion The outcomes that miR-31 was overexpressed in lung cancer，and the difference in accordance with tumor major axis as well as the relationship with cigarettes indicated miR-31 might be a biomarker for screening，diagnosis，and predicting the tumor axis.
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