Abstract: Objective To investigate the relationship of heparanase and Src in gastric carcinoma cells. Methods Western-blot was used to detect pp2 activity，the inhibitor of Src，and its effect on heparanase expression of human gastric carcinoma SGC-7901 cells，and p-Src expression in human gastric carcinoma SGC-7901 cells with silenced Heparanase. Results pp2，the inhibitor of Src，did not affect Heparanase protein expression in human gastric carcinoma SGC-7901 cells，p-Src expression decreased in human gastric carcinoma SGC-7901 cells with silenced Heparanase. Conclusion Heparanase might regulate Src phosphorylation，and Heparanase-Src might be a signal transduction pathway of invasion，migration and angiongenesis of human gastric cancer.