龙泉复方制剂的抑瘤作用及其作用机制
Effect of Chinese Medicine Longquan Fufang on Tumor Growth and Its Mechanism
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摘要: 目的探讨中药验方“龙泉复方制剂”的抑瘤作用及其作用机制。方法Lewis肺癌接种于DBA/2小鼠。S180肉瘤接种于BALB/C小鼠。将各味中药成分煮沸煎制,过滤后备用,每只鼠0.5ml/d,共10次。体外细胞毒性实验:MTT比色法。脾细胞细胞因子mRNA表达水平分析:RT-PCR。结果(1)本方对两种肿瘤都有明显的抑制作用,抑瘤作用与药量大小呈正比。同量的药对Lewis肺癌的作用更强一些。(2)用药后小鼠脾细胞对肿瘤细胞的杀伤率明显高于对照组;随着效靶比例的增加小鼠脾细胞对肿瘤细胞的杀伤率逐渐上升。(3)用药组脾细胞的IL-2和INF-γ的表达水平均明显增高。B7-H1的表达比对照组有所降低,但差异没有统计学意义。结论龙泉复方制剂是一种非常有前景的治疗肿瘤的复方中药,其作用机制可能是,对肿瘤细胞直接的毒性作用和通过促进免疫反应的间接抑瘤作用Abstract: Objective To study the effect of Chinese medicine Longquan Fufang on tumor growth and its mechanism. Methods Lewis Lung cancer line was inoculated subcutaneously in DBA/2 mice. S180 cells was inoculated intramuscularly in BALB/c mice. The supernant of herbs ( 0.5 ml/day, each mouse) was given into mouse stomach for ten times. Cytotoxicity of CTL in vitro was measured with MTT colorimetry. The expression of mRNA was analyzed with RT-PCR. Results Longquan Fufang can inhibit significantly the growth of two can...
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