肿瘤抗原致敏树突状细胞治疗胶质瘤的实验研究
Experimental Study of Vaccination with Dendritic Cells Pulsed with Tumor Cell Lysates for Immunotherapy of C_6 Glioma
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摘要: 目的 研究肿瘤冻融抗原致敏树突状细胞 (DC)治疗胶质瘤的疗效。方法 从大鼠骨髓中分离出单核细胞后, 加入添加rGM CSF和rIL 4的IMDM培养基中培养后鉴定。制备大鼠C6胶质瘤冻融抗原以及DC疫苗, 同时肿瘤冻融抗原致敏DC体外细胞毒试验 ;建立动物模型, 体内应用DC疫苗并观察疗效。结果 单核细胞培养 8天后可获得大量的树突状细胞 ;体外细胞毒试验发现对相应的C6肿瘤细胞有明显的杀伤作用 ;动物实验发现治疗组肿瘤生长明显抑制, 而对照组肿瘤明显生长, 两组肿瘤体积有显著性差异P <0 .0 1。结论 建立了体外扩增大鼠骨髓DC以及制备DC疫苗的方法, 采用冻融抗原致敏DC疫苗治疗荷瘤动物生存时间明显延长, 肿瘤生长明显抑制, 为研究其在脑胶质瘤临床免疫治疗打下基础Abstract: Objective To investigate effect of dendritic cells pulsed with tumor antigens which are used to treat with intracranial glioma. Methods dendritic cells were isolated from rat bone marrow precursors stimulated in vitro with granulocyte macrophage colony stimulating factor (GM CSF) and interleukin 4 (IL 4).Then mature DCs were analyzed; Produce lysates and dendritic cells vaccinum; then we established animal model treated with dendritic cells vaccinum. The rat brains were then removed and examined hist...
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