tk基因治疗脑胶质瘤时的免疫反应与旁观者效应的关系

Relation between immune response and bystander effect when using tk gene to treat therapy glioma

摘要: 目的　探讨tk基因 (thymidinekinasegene)治疗脑胶质瘤时的免疫反应与旁观者效应的关系。方法　将C6 tk+与C6 tk 按 0 %tk+,10 %tk+,30 %tk+,5 0 %tk+,10 0 %tk+5种比例混合, 接种于SD鼠右侧顶叶, 7d后腹腔注射GCV(更昔洛韦 ), 剂量为 30mg/kg/d, 共 10d。细胞接种 3周后, 处死动物, 计算成瘤率, 作病理检查, 并作CD4 +、CD8+的免疫组化染色。结果　 5 0 %及 10 0 %C6 tk+组的成瘤率明显小于其余3组 (P <0 .0 5 ), 病理检查发现 5 0 %及 10 0 %tk+组有淋巴细胞及浆细胞浸润。免疫组化显示每一组之间均有差异, 提示有免疫反应存在。结论　tk基因治疗脑胶质瘤时, 可激起机体内的免疫反应, 增强抗肿瘤作用, 它是旁观者效应的作用机制之一

Abstract: Objective To investigate the relation between immune response and bystander effect when using tk gene for therapy of glioma. Methods tk + and tk - C6 cells were mixed at the ratios of 0% tk +, 10% tk +, 30%tk +, 50%tk +, 100%tk + .The mixed cells were implanted directly to the right parietal lobe of the SD rats. Seven days after cell implanted, all rats were given GCV 30mg/kg/d for 10 days. Three weeks after cell implanted all rats were killed. The rate of tumor growth, the...