Bmi-1基因及其编码蛋白在SKM-1细胞系中的表达

A Study on the Expression of Bmi-1 in SKM-1 Cell Line in vitro

摘要: 目的探讨人类Blymphoma Mo-MLVinsertion region(Bmi-1)基因及其编码蛋白在骨髓增生异常综合征(myelodysplasia syndrome,MDS)发病中的地位和作用。方法以正常骨髓和白血病细胞系K562为对照,利用半定量RT-PCR和Western Blot研究MDS细胞系SKM-1中Bmi-1基因在mRNA和蛋白水平的改变。结果SKM-1中Bmi-1在mRNA和蛋白水平均较正常组织有明显表达(P<0.05),二者表达水平与K562细胞系无显著差异(P>0.05)。结论Bmi-1基因在MDS亚型RAEB细胞系SKM-1中有明显表达,其在RAEB发病中可能具有重要意义。

Abstract: Objective To determine the role of Bmi-1 gene and its coded protein in the incidence of myelodysplasia syndrome. Methods To determine the expression of Bmi-1on the level of mRNA and protein in MDS cell line SKM-1 by means of semi-quantities RT-PCR and Western Blot technique, normal bone marrow and cell line K562 as negative and positive comparison specifically. Results Bmi-1expression level SKM-1 differs from that of normal bone marrow remarkably, while the difference is not remarkable from that of K562. Co...