Abstract: Objective To establish suitable metastatic rabbit Vx|2 liver tumor model for experimental study and to probe into different tumor transplanted methods. Methods 60 male New Zealand white rabbits were divided into 3 groups at random,20 in each group. Vx|2 tumor cells were transplanted into rabbit livers in different way.Then observe :1、the success rate of implanting tumor.2、the volume change of tumor by ultrasonograpy and calculate the growth rate of tumor.3、the biological features of tumor (histopathology and electronic microscope photographs).4、the DSA imagining features of tumor. Results The success rate of transplanting tumor of 3 groups were 7/20、10/20、19/20,the retrofit group was the highest of 3 groups(P<0.05).The tumor grew as exponential curve. Histopathology and electronic microscope photographs indicated that the tumor grow in the liver tissue and infiltrated into the normal tissue.The tumor had the similar biological features of squamous cell carcinoma that transplanted in other sites of rabbits. Conclusion It is successful to set up retrofit metastatic rabbit Vx|2 liver carcinoma model. It is obvious that the success rate of transplanting tumor tissue mass is higher than the other two methods,and it is possible to gain a reliable mature large tumor animal modelfor the basic and clinical study of liver carcinoma intervenient therapy.