Abstract: ObjectiveTo explore the effects of adjuvant chemotherapy combined with Kang'ai injection on the expression of serum VEGF in patients with advanced non-small cell lung cancer. MethodsForty-six patients with advanced non-small cell lung cancer were randomly divided equally into two groups .Patients in experimental group were treated with gemcitabine and cisplatin chemotherapy regimen(GP) combined with Kang'ai injection, while patients in the control were just treated with GP regimen chemotherapy. The expression levels of serum vascular endothelial growth factor(sVEGF) were measured by ELISA before and after the treatment,respectively. ResultsThe sVEGF levels of all patients with advanced NSCLC were obviously higher than that of health controls(P＜0.05). And the level of sVEGF in squamous cell cancer group was higher than that in adenocarcinoma,large cell carcinoma or adenosquamous carcinoma group,respectively(P＜0.05),while there was no significant difference between the latter 3 groups(P＞0.05). Compared the well differentiated group,the sVEGF level of moderately and poorly defferentiated group was increased with no significance(P＞0.05). The sVEGF level was obviously decreased in two groups after the treatments(P＜0.05).Furthermore, the sVEGF level was significantly decreased combination therapy group than that in chemotherapy group(P＜0.05). ConclusionKang'ai injection might decrease the expression of serum VEGF in patients with advanced non-small cell lung cancer which suppressed neovascularization. Serum VEGF could be a biomarker for lung cancer diagnosis and therapeutic effect of chemotherapy or biotherapy.