Analysis of Influence Factors on siRNA Efficacy Silencing mdr1 Gene in SGC7901/VCR Cells

GAO Fu-lian; ZHU Xiao-yan; WANG Feng; WU Jing-lan; ZHANG Qin-xian

doi:10.3971/j.issn.1000-8578.446

GAO Fu-lian, ZHU Xiao-yan, WANG Feng, WU Jing-lan, ZHANG Qin-xian. Analysis of Influence Factors on siRNA Efficacy Silencing mdr1 Gene in SGC7901/VCR CellsJ. Cancer Research on Prevention and Treatment, 2006, 33(03): 151-155. DOI: 10.3971/j.issn.1000-8578.446

Citation:

Analysis of Influence Factors on siRNA Efficacy Silencing mdr1 Gene in SGC7901/VCR Cells

Abstract

Abstract

Objective To analyze the influence factors on siRNA efficacy silencing mdr1 gene in human gastric cancer SGC7901/VCR cells. Methods Four siRNAs(mdr1si326,mdr1si1513,mdr1si2631 and mdr1si3071)specifically targeting mdr1 gene were designed and synthesized by in vitro transcription. The silencing efficacy of the four siRNAs was evaluated with RT-PCR for mdr1 mRNA expression, immunoblotting for P-glycoprotein (P-gp, MDR1) expression, flowcytometry for adriamycin (ADR) accumulation and MTT for sensitivity to ADR...

FullText(HTML)

References (0)

Cited By

Turn off MathJax

Article Contents

Analysis of Influence Factors on siRNA Efficacy Silencing mdr1 Gene in SGC7901/VCR Cells

Abstract

Catalog

Export File

Citation

Format

Content