Effect of Expression of bcl-2 and Apoptosis Induction by Curcumin in Colon Cancer Cell SW480

Objective To study the effect of curcumin on apopsis and bcl-2 expression in SW480 human colon cancer cells. Methods The inhibitive effect of curcumin on SW480 was measured with MTT. Cell cycle phase was analysised by flow cytometry. Apoptosis of SW480 was studied by TUNEL. The expression of bcl-2 gene was determined by immunohistochemical analysis. Results MTT test showed that curcumin was cytotoxic to cells .the number of the apoptosis cell line increased.FCM showed that the proprortion of G_ 0/G_ 1 phase...