Infl uence of BAX on Apoptosis and Drug Sensitivity of A549 Cells

Objective To investigate the role of BAX in apoptosis and response of human A549 cells to apoptosis induced by Cisplatin(DDP). Methods The proliferation of A549 cells were detected by CCK8 calorimetry after treated with Cisplatin. The plasmid containing BAX cDNA was transfected into A549 cell line by lipofectamine transfection. Expression of BAX was detected by RT-PCR. A549, A549/BAX and A549/pcDNA3 were treated with different concentrations of DDP for 72 h. Survival rate of cells were detected by CCK8. Flow cytometry was used to analyze the apoptosis and cell cycle of A549 cells. Results Cisplatin inhibited the proliferation of A549 cells in vitro. BAX and cisplatin retarded A549 cell at G1/S stage. RT-PCR result showed transfection of BAX vector increased BAX mRNA level, and apoptosis of BAX transfection group and cisplatin treatment group were signifi cantly higher than that in control group. Conclusion Stable transfection of BAX signifi cantly enhances the sensitivity of A549 cells to apoptosis induced by Cisplatin.