Relationship between Downregulation of DAL-1 and Carcinogenesis of Colorectal Adenocarcinoma
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摘要: 目的探讨DAL-1表达下调与大肠腺癌发生发展的关系。方法RT-PCR检测6株大癌细胞系和51例组织标本DAL-1 mRNA表达；BSP和MSP法分别检测大肠癌细胞系和组织标本DAL-1基因启动子甲基化状态；5-aza-dC作用实验验证大 肠癌细胞系中DAL-1启动子甲基化情况。 结果6株大肠癌细胞系DAL-1均有表达，各细胞系间表达无明显差异；部分大肠腺癌组织中DAL-1表达下调，且在淋巴结转移患者和Dukes C、D期患者更常见（P＜0.05）。DAL-1基因启动子高甲基化是少见现象。结论DAL-1表达下调在大肠腺癌发生发展中发挥重要作用，特别是在进展期大肠腺癌；但该基因启动子高甲基化不是下调其表达的主要机制。Abstract: ObjectiveTo study the relationship of DAL-1 downregulation and carcinogenesis of colorectal adenocarcinoma. Methods RT-PCR was used to detect DAL-1 mRNA expression in colorectal cancer cell lines and colorectal adenocarcinoma tissues.BSP and MSP were performed to examine the DAL-1 promoter methylation status in colorectal cancer cell lines and colorectal adenocarcinoma tissues.5-aza-dC treatment was performed to verify the promoter methylation status of DAL-1 in colorectal cancer cell lines. Results Expression of DAL-1 was found in colorectal cancer cell lines; there was no significant difference between DAL-1 expressions among the cell lines.DAL-1 was significantly reduced in a portion of colorectal adenocarcinoma tissues.Frequency of DAL-1 downregulation was significantly higher in patients with lymph node metastasis or in Dukes C and Dukes D (P＜0.05).DAL-1 promoter methylation was rare phenomenon. Conclusion Downregulation of DAL-1 plays an important role in carcinogenesis of colorectal adenocarcinoma, especially in advanced colorectal adenocarcinoma.However, the gene promoter methylation doses not regulate the expression of DAL-1.
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Key words:
- DAL-1 gene /
- Colorectal adenocarcinoma /
- Methylation
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