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CHEN Zeng, LIAO Jinrong, ZOU Changyan, SU Ying, LIN Keyu, JIN Shanfeng, ZHENG Qianlan, LIN Xiandong. Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2021, 48(9): 846-852. DOI: 10.3971/j.issn.1000-8578.2021.21.0383
Citation: CHEN Zeng, LIAO Jinrong, ZOU Changyan, SU Ying, LIN Keyu, JIN Shanfeng, ZHENG Qianlan, LIN Xiandong. Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells[J]. Cancer Research on Prevention and Treatment, 2021, 48(9): 846-852. DOI: 10.3971/j.issn.1000-8578.2021.21.0383

Expression of circ_0006692 in Non-small Cell Lung Cancer and Its Regulatory Mechanism on Proliferation and Metastasis of Lung Cancer Cells

  • Objective To explore the expression of circ_0006692 in NSCLC and its relation with clinicopathological characteristics and related mechanism.
    Methods We collected 50 pairs of NSCLC tissues and adjacent tissues. The expression of circ_0006692 was detected by qRT-PCR and its relation with clinicopathological features was analyzed. We constructed lung cancer A549 cell line with circ_0006692 overexpression and knockdown. Cell proliferation, migration and invasion were detected by MTS, colony forming, wound healing and Transwell invasion assays. qRT-PCR and Western blot were used to detect the effect of circ_0006692 expression change on EMT-related gene expression.
    Results The expression of circ_0006692 in NSCLC was significantly higher than that in adjacent tissues (P < 0.05). The expression level of circ_0006692 was closely related to tumor size, TNM stage and pulmonary membrane invasion (P < 0.05). circ_0006692 knockdown inhibited cell proliferation, invasion and metastasis, while its overexpression promoted cell proliferation, invasion and metastasis. circ_0006692 knockdown inhibited the expression of EMT-related genes CDH2 and MMP7 in A549 cells and promoted the expression of CDH1.
    Conclusion The upregulated expression of circ_0006692 in NSCLC is closely related to tumor size, TNM stage and pulmonary membrane invasion. circ_0006692 expression could regulate the proliferation, invasion, metastasis and EMT progression of lung cancer A549 cells.
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